Based on this technique, a complex peptide mixture is prepared from a protein sample and loaded onto a triphasic microcapillary column directly, which is packed with reversed phase, reversed phase HPLC grade material and strong cation exchange. As long as the complex peptide mixture is loaded onto the triphasic microcapillary column, this column will be placed directly in-line with a tandem mass spectrometer. And then the tandem mass spectrometry data generated from a MudPT run will be searched to determine the protein content of the original sample.
Multidimensional protein identification technology is now a widely and powerful accepted approach for protein identification for dealing with a wide variety of samples. It also has been proven to be an excellent tool for both qualitative and quantitative proteomic analysis. Based on this technique, complex peptides mixtures can be well separated through on-line two dimensional HPLC. Recent researches show that better results can be achieved by using MudPT for protein identification, even for a relatively simple sample. These advantages are not existed in traditional methods, such as single dimension LC method.
As a popular method in protein identification category, multidimensional protein identification technology allows scientists to gain valuable and accurate results through advanced techniques.